Taking DNA from Strawberries in the Lab

Taking DNA from Strawberries in the Lab

Table of Contents

Order ID	53003233773
Type	Essay
Writer Level	Masters
Style	APA
Sources/References	4
Perfect Number of Pages to Order	5-10 Pages

Description/Paper Instructions

 

Taking DNA from Strawberries in the Lab

Background 1: Extracting DNA from Strawberries
You will extract DNA from strawberries in this project. You’ll crush strawberries in soapy water for the initial step of the technique. The strawberry cells’ nuclear and cell membranes will be broken apart (lysed) by the soap. The DNA, along with the rest of the cell’s contents, will pour out once the nuclear and cell membranes have been disrupted. The DNA will disintegrate in the available water. In the second step of the operation, you’ll add isopropanol to the aqueous solution to make a solvent mixture that’s less soluble for the DNA. As a result, the DNA will clump together and “crash” out of the solution as a solid. The DNA will then be “fished out” with a paper clip.
• 2 teaspoons liquid dish soap • 12 cup tap water • two plastic cups • coffee filter or paper towel • rubber band • 14 to 12 cup COLD rubbing alcohol (70 percent or 91 percent isopropanol or isopropyl alcohol, refrigerated in refrigerator)
Submission Remarks
This lab should be written in your composition book. Make a list of any observations you make, as well as your responses to questions. To organize the many components of the lab, use headers. As advised, take photos of lab items as well as your written pages. Make a single pdf file out of all of these photographs and upload it to the Canvas assignment for this lab.

2 StrawberryExtractionInstructions.pdf adapted from https://www.genome.gov/Pages/Education/Modules/StrawberryExtractionInstructions.pdf
1. Remove the leaves from two or four large or small strawberries.
2. Put the strawberries in a Ziploc bag, seal it, and smash it lightly for two minutes.
The objective is to smash the strawberries while avoiding breaking the bag.
3. Combine a half cup of tap water, one teaspoon of salt, and two teaspoons of liquid dish soap in your first plastic cup. Stir.
2. Pour two teaspoons of the dish soap mixture into the bag of crushed strawberries.
(Set aside the first cup, which contains the dish soap mixture.) You won’t require it any more.)
After that, close the bag and gently compress the contents for another minute. Break the bag as little as possible, and don’t make a lot of soap bubbles.
5. Line the opening of your second plastic cup with a coffee filter or a piece of paper towel. Make a trough out of a paper towel and fasten it to the cup by wrapping a rubber band around the corners that are folded over the cup’s mouth.
6. Strain the soapy strawberry slush through a paper towel or a filter. GENTLY push the sludge with the round part of a spoon or your fingertips to get the liquid to pass through the filter or paper towel into the cup below. Breaking the filter or the paper towel is not a good idea. You can squeeze the filter or paper towel by lifting it out of the cup, but be careful! The filter or paper towel should not be torn or poked. The idea is to catch the strawberry sludge in the filter or paper towel and let the liquid flow into the cup below.
7. Remove the paper towel or filter from the cup. Pour cold rubbing alcohol down the side of the cup slowly while tilting it at a 45-degree angle. Pour in the same amount of rubbing alcohol as the strawberry extract liquid already in the cup. (It’s possible that this is a small volume.) On top of the strawberry extract layer, a layer of rubbing alcohol must be formed. Allow the layers to remain separate. Do not agitate or disturb the layers.
8. A white, hazy substance (DNA) will appear in the top layer above the strawberry extract layer after a few minutes.
9. Make a paper clip straight. Then, at the end of the paper clip, make a hook. After that, dip the hook into the cup and pull out the stringy, gooey DNA. You might find it easier to do this if you tilt the cup. Take a selfie with the DNA you fished out with the paper clip hook. Include this photograph with your lab submission.

3 Follow-up Questions
1. The nuclear membrane/envelope (the membrane that surrounds the cell’s nucleus) has a structure that is similar to the cell membrane. What causes the nuclear and cell membranes to be disrupted by soap? Consider the similarities and differences between the structure of soap and the structure of a cell membrane. In your explanation, include descriptions of these structures. You may include a drawing to accompany your explanation if desired.
2. Which feature of DNA’s structure makes it water soluble? What are the intermolecular forces that exist between water and DNA?
3. Why is isopropanol less soluble in DNA than water? Isopropanol (also known as isopropyl alcohol or 2-propanol) has the following structure.

 

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